MICROSCOPIC AND MOLECULAR DETECTION OF NOSEMA SP. IN THE SOUTHWEST AEGEAN REGION

Beekeeping, performed in many parts of the world, has a very large place world trade market with bee products such as wax, venom, propolis and royal jelly, especially honey production. However, diseases are quite common restricted production products. One most important these diseases, Nosema, is caused by spores intestinal epithelium cells honeybee. Nosema apis ceranae factors this disease also our country. These two species can be distinguished from each other molecular diagnostic methods. In study, materials collected 152 apiaries located 13 districts Muğla province 62 water sources close to apiaries. The were counted using Thoma lame under light microscope. DNA isolation was carried out spore positive samples. 218MITOC FOR-REV 321APIS primers used figure N. species. After sequence analysis obtained amplifications, it determined that all samples formed 3 haplotypes according studied sequences for first time. region, presence only agent prevalence detected at rate 71.53±6.02%. Moreover, blast showed high similarity (94-100 %) previously reported Lebanon, France, Morocco Thailand